Nitrofurantoin (AHD) ELISA Test Kit
Product Description
REAGEN™Nitrofurantoin (AHD) ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of nitrofurantoin in fish, shrimp, meat (chicken, beef, pork, and hepar), eggs, and honey.
The unique features of the kit are:
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Rapid (4 hours), high recovery (80% - 105%), and cost-effective sample extraction methods.
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High sensitivity (0.03 ng/g or ppb) and low detection limit (0.06 ng/g or ppb) for various samples.
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High reproducibility.
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A quick ELISA assay (less than 1 hour regardless of several samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The AHD-OVA has been coated in the plate wells. During the analysis, the sample and the AHD antibody are added along with a secondary antibody, tagged with a peroxidase enzyme. If the AHD residue is present in the sample, it will compete for the AHD antibody, thereby preventing it from binding to the AHD-OVA attached to the well. After adding the HRP substrate (TMB), the resulting color intensity has an inverse relationship with the AHD residue concentration in the sample.
Kit Contents, Storage, and Shelf Life
REAGEN™Nitrofurantoin (AHD) ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| AHD -Coated Plate | 1 x 96well plate (8 wells x 12strips) | 2-8°C |
| AHD Standards: Negative control (white cap tube) 0.03 ng/mL (yellow cap tube) 0.1 ng/mL (orange cap tube) 0.4 ng/mL (pink cap tube) 1 ng/mL (purple cap tube) 4 ng/mL (blue cap tube) 10 ng/mL(spiking,optional, red cap tube) | 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL | 2-8°C 2-8°C |
| AHD Antibody (Antibody#1) | 8 mL | 2-8°C |
| HRP-Conjugated Antibody #2 | 6 mL |
| 10X Sample Extraction Buffer | 25 mL |
| 20X Wash Solution | 30 mL |
| Stop Buffer | 12 mL |
| TMB Substrate | 12 mL |
| 50 mM 2-Nitrobenzaldehyde | 2.0 mL |
If you are not planning to use the kit for over 1 month, store Antibody#1 and HRP-Conjugated Antibody #2 at -20°C or in a freezer.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Fish/Shrimp/Meat | 0.06 |
| Meat (chicken,beef,pork and hepar) | 0.06 |
| Egg/Egg Powder | 0.06 |
| Honey | 0.06 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| AHD | 100 |
| AOZ | <0.04 |
| SEM | < 0.06 |
| AMOZ | < 0.05 |
Product Tags:
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Rapid Nitrofurantoin (AHD) ELISA Test Kit High Recovery Low Detection Limit Images
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