Total Aflatoxin ELISA Test Kit
Product Description
REAGEN™Total Aflatoxin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Total Aflatoxin in cereals, meat/fish, feed, milk ,oils,peanuts and pistachios.
The unique features of the kit are:
1. High recovery (80-105%),rapid(10-40minutes), cost-effective extraction methods.
2. High sensitivity (0.05ng/g or ppb).
3. High reproducibility.
4. A quick ELISA assay (less than 2 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The second antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the toxin coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Meat/fish/sereal/feed/seed/starch | 0.5 |
| Meat/liver/kidney | 0.5 |
| Milk | 0.5 |
| Milk powder | 4.5 |
| Peanut butte | 0.5 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Aflatoxin B1 | 100.0 |
| Aflatoxin B2 | 76 |
| Aflatoxin M1 | 79 |
| Aflatoxin M2 | 33 |
| Aflatoxin G1 | 55 |
| Aflatoxin G2 | 6 |
Warnings and Precautions
§ The standards contain Total Aflatoxin . Handle with particular care.
§ Do not use the kit past the expiration date.
§ Do not intermix reagents from different kits or lots except for components with the same part No’s within their expiration dates.
§ Try to maintain a laboratory temperature of 20°–25°C (68°–77°F). Avoid running assays under or near air vents, as this may cause excessive cooling, heating and/or evaporation. Also, do not run assays in direct sunlight, as this may cause excessive heat and evaporation. Cold bench tops should be avoided by placing several layers of paper towel or some other insulation material under the assay plates during incubation.
§ Make sure you are using only distilled or deionized water since water quality is very important.
§ When pipetting samples or reagents into an empty microtiter plate, place the pipette tips in the lower corner of the well, making contact with the plastic.
§ Incubations of assay plates should be timed as precisely as possible. Be consistent when adding standards to the assay plate. Add your standards first and then your samples.
§ Add standards to plate only in the order from low concentration to high concentration as this will minimize the risk of compromising the standard curve.
§ Always refrigerate plates in sealed bags with a desiccant to maintain stability. Prevent condensation from forming on plates by allowing them equilibrate to room temperature (20 – 25°C / 68 – 77°F) while in the packaging.
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